Xmipp

xmipp_ctf_group (v3.0)

Usage

Generate CTF (or defocus) groups from a single CTFdat file. The automated mode groups all images with absolute difference in CTF-values up to a given resolution below a given threshold. For example the example bellow groups all images together with absolute CTF differences smaller than 0.5 up to 15 Angstroms resolution). A complementary manual mode allows to combine different groups or to split) groups up even further.

See also
ctf_create_ctfdat

Parameters

--ctfdat <ctfdat_file>
Input CTFdat file for all data
-o <oext=ctf>
Output root name, you may force format ctf:mrc
--pad <float=1>
Padding factor
--phase_flipped
Output filters for phase-flipped data
--discard_anisotropy
Exclude anisotropic CTFs from groups
--wiener
Also calculate Wiener filters
--memory <double=1.>
Available memory in Gb
--do1Dctf
Compute Groups using 1D CTF, select this option is you have many non astismatic CTFs
--wc <float=-1>
Wiener-filter constant (if < 0: use FREALIGN default)

MODE 1: AUTOMATED:

--error <float=0.5>
Maximum allowed error
--resol <float=-1>
Resol. (in Ang) for error calculation. Default (-1) = Nyquist

MODE 2: MANUAL:

--split <docfile>
1-column docfile with defocus values where to split the data

Examples and notes

Example of use: Sample using automated mode (resolution = 15 Ang.)
   xmipp_ctf_group --ctfdat all_images_new.ctfdat -o CtfGroupsNew/ctfAuto   --wiener --wc -1 --pad 2 --phase_flipped --error 0.5 --resol 15 
Example of use: Sample using manual mode (after manual editing of ctf_group_split.doc)
   xmipp_ctf_group --ctfdat all_images_new.ctfdat -o CtfGroupsNew/ctfManual --wiener --wc -1 --pad 2 --phase_flipped --split CtfGroupsNew/ctf_group_split.doc

User's comments

%COMMENT{type="tableappend"}%